Estimation Of - Lipid Profile ( Total Serum Cholesterol, TG, LDL, HDL )

Reagent:- Constant 

Procedure Of - Total Serum Cholesterol:-

1000 µl Reagent + 10 µl Serum 
                        +
          Wait for 10 minute
                       +
  Then Reading on analyzer

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Procedure Of - TG :-

1000 µl TG Reagent + 10 µl Serum .
                        +
          Wait for 10 minute.
                       +
  Then Reading on analyzer.

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Procedure Of - HDL :-

1. 1000 µl HDL Reagent + 500 µl Serum .
2.  Centrifused 10 minute.
3. Take 100  µl Supernatant from centrifuged solution.
4. Add 1000 µl Cholesterol Reagent.
5. Wait for 10 minute 
6. Then Reading on Analyzer.  

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 LDL = Total Cholesterol - ( TG / 5 + HDL )
                           OR
Procedure Of - LDL :-

1000 µl LDL Reagent + 20 µl Serum .
                        +
          Wait for 10 minute .
                       +
  Then Reading on analyzer.