Estimatation Of - SGPT

Reagent:-

1.  SGPT Color Reagent.
2. DNPH 
3. NaoH

Manual:-
Procedure:-

1. Color Reagent 100 µl  + Serum 20 µl 
2. Wait 30 minute at 37 degree temperature.
3. DNPH 100 µl .
4. Wait for 20 min at room temperature.
5. NaoH 1000 µl.
6. Result at colorimeter

Kinetic:-
Procedure:-
        Reagent 1000 µl  + Serum 100 µl  
                                  +
        Then Reading on auto analyzer