Estimation Of - Serum T3 In Biochemistry Laboratory
Estimation Of - Serum T3 In Laboratory:-
Procedure Of- Wash Buffer For T3
1 Part Wash Buffer
19 Part Distilled Water
Or 100 µl Wash Buffer and 1900 µl D/W and mixed well
Procedure Of - Conjugate For Hormone Test:-
Take 1 Part Concentrated Conjugate With 10 Part Diluted Conjugate . For eg: concentrate conjugated 10 µl and Diluted conjugate 100 µl and mixed well. ( its just 1 test ). Solution made up just after 20 minutes of test.
Procedure:-
Md. Safiqul Islam Babu
BSC in Laboratory Medicine
Dhaka University 2014
Saic Institute Of Medical Technology
Procedure Of- Wash Buffer For T3
1 Part Wash Buffer
19 Part Distilled Water
Or 100 µl Wash Buffer and 1900 µl D/W and mixed well
Procedure Of - Conjugate For Hormone Test:-
Take 1 Part Concentrated Conjugate With 10 Part Diluted Conjugate . For eg: concentrate conjugated 10 µl and Diluted conjugate 100 µl and mixed well. ( its just 1 test ). Solution made up just after 20 minutes of test.
Procedure:-
- Take 50 µl Patient Serum + Conjugate 100 µl + Antibody 50 µl ( Shaking 6 Sec )
- Wait For 60 Minute .
- Then Wash 6 Times With Wash Buffer .
- Add Substrate 100 µl ( Shaking 6 Sec ).
- Wait 20 Minute In The Dark Place.
- Then Add Stop Solution 100 µl .
- Then Reading On Analyzer .
Md. Safiqul Islam Babu
BSC in Laboratory Medicine
Dhaka University 2014
Saic Institute Of Medical Technology
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ReplyDeleteDetailed SOP-style write-up — the wash-buffer discipline (6 washes, no shortcuts) is exactly the documentation habit that keeps immunoassays reproducible. We apply the same discipline to our reference material paperwork: every peptide standard ships with its analytical documentation, e.g. https://yourfitnesspeptides.com/product/buy-selank-10mg/ — batch COA with HPLC purity data, so the lab can verify identity and purity before the material ever touches an assay. — Asher Gray (YourFitnessPeptides team; laboratory research use only)
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