Estimation Of - Serum Testosterone In Biochemistry Laboratory

Estimation Of - Serum Testosterone:-

Procedure:- 

Take 50 µl Patient Serum + Conjugate 100 µl + Antibody 50 µl ( Shaking 6 Sec )
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                                            Wait For 90 Minute .
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                              Then Wash 6 Times With Wash Buffer.
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                             Add Substrate 100 µl  (  Shaking 6 Sec ).
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                               Wait 20 Minute In The Dark Place. 
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                                   Then Add Stop Solution  100 µl . 
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                                       Then Reading On Analyzer .